InhaltsangabePart I. Design Strategies for Synthetic Gene Network 1. In silico Implementation of Synthetic Gene Networks            Maria Andrea Marchisio 2. Standardization in Synthetic Biology            Kristian M. Müller and Katja M. Arndt 3. Robust Optimal Design of Synthetic Biological Networks            Dirk Lebiedz, Marcel Rehberg, and Dominik Skanda 4. Predicting Synthetic Gene Networks            Diego di Bernardo, Lucia Marucci, Filippo Menolascina, and Velia Siciliano  Part II. Development and Characterization of the Synthetic Biology Toolbox 5. Reprogramming a GFP Reporter Gene Subjects it to Complex Lentiviral Gene Regulation            Frank Notka and Ralf Wagner 6. A High-throughput Microfluidic Method for Generating and Characterizing Transcription Factor Mutant Libraries            Marcel Geertz, Sylvie Rockel, and Sebastian J. Maerkl 7. Identifying and Optimizing Intracellular Protein-protein Interactions using Bacterial Genetic Selection            Dujduan Waraho and Matthew P. DeLisa 8. Zinc-finger Nucleases Based Genome Engineering to Generate Isogenic Human Cell Lines            Anne-Kathrin Dreyer and Toni Cathomen 9. RNA-based Networks: Using RNA Aptamers and Ribozymes as Synthetic Genetic Devices            Julia E. Weigandm Alexander Wittmann, and Beatrix Suess 10. MicroRNA Circuits for Transcriptional Logic            Madeleine Leisner, Leonidas Bleris, Jason Lohmueller, Zhen Xie, and Yaakov Benenson 11. Light-regulated Gene Expression in Yeast            Laszlo Kozma-Bognar, Anita Hajdu, and Ferenc Nagy 12. Light-controlled Gene Switches in Mammalian Cells            Fuzhong Zhang, Kristian M. Müller, G. Andrew Woolley, and Katja M. Arndt 13. Expressed Protein Modifications: Making Synthetic Proteins            Birgit Wiltschi Part III. Optimizing the Host Systems 14. Using Transcription Machinery Engineering to Elicit Complex Cellular Phenotypes            Amanda M. Lanza and Hal S. Alper 15. Streamlining of a Pseudomonas putida Genome using a Combinatorial Deletion Method Based on Mini-transposon Insertion and the Flp-FRT Recombination System            Audrey Leprince, Danielle Janus, Victor de Lorenzo, and Vitor Martins dos Santos 16. Transposon-based and Plasmid-based Genetic Tools for Editing Genomes of Gram-negative Bacteria            Esteban Martinez-García and Víctor de Lorenzo  Part IV. Implementation of Synthetic Gene Networks in Different Host Systems 17. Synthetic Networks: Oscillators and Toggle Switches for Escherichia coli            Nicolas Perry and Alexander J. Ninfa 18. Studying Microbial Communities in Biofilms            Katie Brenner 19. Quantitative Analysis of the Spatiotemporal Dynamics of a Synthetic Predator-prey Ecosystem            Stephen Payne, Robert Phillip Smith, and Lingchong You 20. Drosophila S2 Schneider Cells - A Useful Tool for Rebuilding and Redesigning Approaches in Synthetic Biology            Jianying Yang and Michael Reth 21. Synthetic Gene Networks in Plant Systems            Astrid Junker and Björn H. Junker 22. Design and Construction of Synthetic Gene Networks in Mammalian Cells            Maria Karlsson, Wilfried Weber, Martin Fussenegger 23. Synthetic Gene Networks as Blueprint for Smart Hydrogels            Michael M. Kämpf and Wilfried Weber

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Part I. Design Strategies for Synthetic Gene Network   1. In silico Implementation of Synthetic Gene Networks             Maria Andrea Marchisio   2. Standardization in Synthetic Biology             Kristian M. Müller and Katja M. Arndt   3. Robust Optimal Design of Synthetic Biological Networks             Dirk Lebiedz, Marcel Rehberg, and Dominik Skanda   4. Predicting Synthetic Gene Networks             Diego di Bernardo, Lucia Marucci, Filippo Menolascina, and Velia Siciliano     Part II. Development and Characterization of the Synthetic Biology Toolbox   5. Reprogramming a GFP Reporter Gene Subjects it to Complex Lentiviral Gene Regulation             Frank Notka and Ralf Wagner   6. A High-throughput Microfluidic Method for Generating and Characterizing Transcription Factor Mutant Libraries             Marcel Geertz, Sylvie Rockel, and Sebastian J. Maerkl   7. Identifying and Optimizing Intracellular Protein-protein Interactions using Bacterial Genetic Selection             Dujduan Waraho and Matthew P. DeLisa   8. Zinc-finger Nucleases Based Genome Engineering to Generate Isogenic Human Cell Lines             Anne-Kathrin Dreyer and Toni Cathomen   9. RNA-based Networks: Using RNA Aptamers and Ribozymes as Synthetic Genetic Devices             Julia E. Weigandm Alexander Wittmann, and Beatrix Suess   10. MicroRNA Circuits for Transcriptional Logic             Madeleine Leisner, Leonidas Bleris, Jason Lohmueller, Zhen Xie, and Yaakov Benenson   11. Light-regulated Gene Expression in Yeast             Laszlo Kozma-Bognar, Anita Hajdu, and Ferenc Nagy   12. Light-controlled Gene Switches in Mammalian Cells             Fuzhong Zhang, Kristian M. Müller, G. Andrew Woolley, and Katja M. Arndt   13. Expressed Protein Modifications: Making Synthetic Proteins             Birgit Wiltschi   Part III. Optimizing the Host Systems   14. Using Transcription Machinery Engineering to Elicit Complex Cellular Phenotypes             Amanda M. Lanza and Hal S. Alper   15. Streamlining of a Pseudomonas putida Genome using a Combinatorial Deletion Method Based on Mini-transposon Insertion and the Flp-FRT Recombination System             Audrey Leprince, Danielle Janus, Victor de Lorenzo, and Vitor Martins dos Santos   16. Transposon-based and Plasmid-based Genetic Tools for Editing Genomes of Gram-negative Bacteria             Esteban Martinez-García and Víctor de Lorenzo     Part IV. Implementation of Synthetic Gene Networks in Different Host Systems   17. Synthetic Networks: Oscillators and Toggle Switches for Escherichia coli             Nicolas Perry and Alexander J. Ninfa   18. Studying Microbial Communities in Biofilms             Katie Brenner   19. Quantitative Analysis of the Spatiotemporal Dynamics of a Synthetic Predator-prey Ecosystem             Stephen Payne, Robert Phillip Smith, and Lingchong You   20. Drosophila S2 Schneider Cells - A Useful Tool for Rebuilding and Redesigning Approaches in Synthetic Biology             Jianying Yang and Michael Reth   21. Synthetic Gene Networks in Plant Systems             Astrid Junker and Björn H. Junker   22. Design and Construction of Synthetic Gene Networks in Mammalian Cells             Maria Karlsson, Wilfried Weber, Martin Fussenegger   23. Synthetic Gene Networks as Blueprint for Smart Hydrogels             Michael M. Kämpf and Wilfried Weber