Inhaltsangabe1. Designing Hairpin-Based RNAi Shuttles            Yu Shen 2. Rapid Cloning and Validation of MicroRNA Shuttle Vectors:  A Practical Guide            Ryan L. Boudreau, Sara E. Garwick-Coppens, Jian Liu, Lindsay M. Wallace, and Scott Q. Harper 3. The Use of Small Non-Coding RNAs to Silence Transcription in Human Cells            Kevin V. Morris 4. Delivery Strategies for RNAi to the Nervous System            Kevin D. Foust and Brian K. Kaspar 5. Cloning Small RNAs            Eric J. Devor and Lingyan Huang 6. Profiling the miRNome: Detecting Global miRNA Expression Levels with DNA Microarrays            Peter White 7. High Throughput Profiling of Mature MicroRNA by Real-Time PCR            Jinmai Jiang, Eun Joo Lee, Melissa Piper Hunter, Clay B. Marsh, and Thomas D. Schmittgen 8. In Situ Detection of MicroRNAs in Paraffin Embedded, Formalin Fixed Tissues: Different Methodologies and Co-Localization with Possible Targets            Gerard J. Nuovo, Patrick Nana-Sinkam, Melissa Crawford, and Thomas D. Schmittgen 9. Tracing of siRNAs Inside Cells by FRET Imaging            Markus Hirsch, Il-Han Kim, Anne Järve, Roger Fischer, Michael F. Trendelenburg, Ulrich Massing, Karl Rohr, and Mark Helm 10. Application of RIP-Chip for the Identification of miRNA Targets            Lu Ping Tan, Anke van den Berg, and Joost L. Kluiver

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1. Designing Hairpin-Based RNAi Shuttles             Yu Shen   2. Rapid Cloning and Validation of MicroRNA Shuttle Vectors:  A Practical Guide             Ryan L. Boudreau, Sara E. Garwick-Coppens, Jian Liu, Lindsay M. Wallace, and Scott Q. Harper   3. The Use of Small Non-Coding RNAs to Silence Transcription in Human Cells             Kevin V. Morris   4. Delivery Strategies for RNAi to the Nervous System             Kevin D. Foust and Brian K. Kaspar   5. Cloning Small RNAs             Eric J. Devor and Lingyan Huang   6. Profiling the miRNome: Detecting Global miRNA Expression Levels with DNA Microarrays             Peter White   7. High Throughput Profiling of Mature MicroRNA by Real-Time PCR             Jinmai Jiang, Eun Joo Lee, Melissa Piper Hunter, Clay B. Marsh, and Thomas D. Schmittgen   8. In Situ Detection of MicroRNAs in Paraffin Embedded, Formalin Fixed Tissues: Different Methodologies and Co-Localization with Possible Targets             Gerard J. Nuovo, Patrick Nana-Sinkam, Melissa Crawford, and Thomas D. Schmittgen   9. Tracing of siRNAs Inside Cells by FRET Imaging             Markus Hirsch, Il-Han Kim, Anne Järve, Roger Fischer, Michael F. Trendelenburg, Ulrich Massing, Karl Rohr, and Mark Helm   10. Application of RIP-Chip for the Identification of miRNA Targets             Lu Ping Tan, Anke van den Berg, and Joost L. Kluiver